| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gao, Sophia (9)
|
ADCPP |
40.83 |
40.34 |
-0.49 |
| 2 |
Carsillo, Ava C (9)
|
ADDELM |
42.16 |
41.95 |
-0.21 |
| 3 |
McMahon, Elizabeth M (10)
|
ADUN |
42.66 |
43.31 |
0.65 |
| 4 |
Harpootlian, Hannah R (10)
|
ADCPP |
44.09 |
43.80 |
-0.29 |
| 5 |
Silaika, Genevieve E (10)
|
ADCPP |
42.17 |
44.20 |
2.03 |
| 6 |
Hao, Helen Z (10)
|
ADCPP |
42.67 |
44.55 |
1.88 |
| 7 |
Donofrio, Sophia B (9)
|
ADCPP |
47.90 |
47.17 |
-0.73 |
| 8 |
Fiacco, Briella P (10)
|
ADBFIN |
49.87 |
48.65 |
-1.22 |
| 9 |
Bourassa, Annalise (10)
|
ADCPP |
|
49.95 |
- |
| 10 |
Zhang, Chloe H (9)
|
ADCPP |
52.63 |
54.31 |
1.68 |
| 11 |
Ensminger, Annabelle G (10)
|
ADCPP |
57.26 |
1:00.28 |
3.02 |
| 12 |
Sharghi, Ava (10)
|
ADCPP |
1:01.88 |
1:01.91 |
0.03 |
| 13 |
Robles, Isabella M (9)
|
ADDELM |
1:04.65 |
1:02.75 |
-1.90 |
| 14 |
Russo, Natalie L (10)
|
ADCPP |
59.97 |
1:06.25 |
6.28 |
| 16 |
Huang, Xiangyi (10)
|
ADCPP |
|
1:12.70 |
- |
| 17 |
Buchakjian, Lola R (9)
|
ADCPP |
1:06.87 |
1:13.14 |
6.27 |
| 18 |
Belyansky, Sophia M (10)
|
ADCPP |
|
1:15.64 |
- |
| 19 |
Murray, Aidan M (9)
|
ADCPP |
|
1:19.36 |
- |
|
Zhu, Ziyu (9)
|
ADCPP |
|
DQ |
- |
|
Halpin, Ella P (9)
|
ADCPP |
57.93 |
NS |
- |